Natural sweet protein monellin has a high sweetness and low calorie, suggesting its potential in food applications. However, due to its low heat and acid resistance, the application of monellin is limited. In this study, we show that the thermostability of monellin can be improved with no sweetness decrease by means of sequence, structure analysis, and site-directed mutagenesis. We analyzed residues located in the α-helix as well as an ionizable residue C41. Of the mutants investigated, the effects of E23A and C41A mutants were most remarkable. The former displayed significantly improved thermal stability, while its sweetness was not changed. The mutated protein was stable after 30 min incubation at 85°C. The latter showed increased sweetness and slight improvement of thermostability. Furthermore, we found that most mutants enhancing the thermostability of the protein were distributed at the two ends of α-helix. Molecular biophysics analysis revealed that the state of buried ionizable residues may account for the modulated properties of mutated proteins. Our results prove that the properties of sweet protein monellin can be modified by means of bioinformatics analysis, gene manipulation, and protein modification, highlighting the possibility of designing novel effective sweet proteins based on structure-function relationships.
from #Medicine via ola Kala on Inoreader http://ift.tt/22TywfY
via IFTTT
Αρχειοθήκη ιστολογίου
-
►
2023
(138)
- ► Φεβρουαρίου (74)
- ► Ιανουαρίου (64)
-
►
2022
(849)
- ► Δεκεμβρίου (61)
- ► Σεπτεμβρίου (74)
- ► Φεβρουαρίου (65)
-
►
2021
(2936)
- ► Δεκεμβρίου (59)
- ► Σεπτεμβρίου (180)
- ► Φεβρουαρίου (325)
-
►
2020
(1624)
- ► Δεκεμβρίου (293)
- ► Σεπτεμβρίου (234)
- ► Φεβρουαρίου (28)
-
►
2019
(13362)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (5586)
- ► Ιανουαρίου (5696)
-
►
2018
(66471)
- ► Δεκεμβρίου (5242)
- ► Σεπτεμβρίου (5478)
- ► Φεβρουαρίου (4835)
- ► Ιανουαρίου (5592)
-
►
2017
(44259)
- ► Δεκεμβρίου (5110)
- ► Σεπτεμβρίου (5105)
-
▼
2016
(7467)
- ► Δεκεμβρίου (514)
- ► Σεπτεμβρίου (1038)
- ► Φεβρουαρίου (793)
-
▼
Ιανουαρίου
(578)
-
▼
Ιαν 10
(24)
- Protective effect of Rhei Rhizoma on reflux esopha...
- Generating Primary Fibroblast Cultures from Mouse ...
- Folate Deficiency Affects Histone Methylation
- Cortical gray matter loss in schizophrenia: Could ...
- Adhesive capsulitis: An age related symptom of met...
- The Baby Food Hypothesis: An explanation why high ...
- Muscle growth across a variety of exercise modalit...
- In situ ionic liquid dispersive liquid–liquid micr...
- FTIR spectroscopy characterization of fatty-acyl-c...
- Precision mixology challenge
- Rapid assessment of singlet oxygen-induced plasma ...
- Sensitive detection of cyanide using bovine serum ...
- Multi-mycotoxin stable isotope dilution LC-MS/MS m...
- E-Health—a topic for analytical chemists?
- Development of gold nanoparticles-based aptasensor...
- Nicholas Long and Wing-Tak Wong: The chemistry of ...
- Quantitative profiling of oxylipins in plasma and ...
- One-pot green hydrothermal synthesis of fluorescen...
- Solution to rubbery egg challenge
- Development of a label-free gold nanoparticle-base...
- Experienced versus Inexperienced Interexaminer Rel...
- Intraoral Digital Impressions for Virtual Occlusal...
- Modification of the Sweetness and Stability of Swe...
- Extraction of Oleic Acid from Moroccan Olive Mill ...
-
▼
Ιαν 10
(24)
Αναζήτηση αυτού του ιστολογίου
Κυριακή 10 Ιανουαρίου 2016
Modification of the Sweetness and Stability of Sweet-Tasting Protein Monellin by Gene Mutation and Protein Engineering
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
-
Αλέξανδρος Γ. Σφακιανάκης Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,0030693260717...
-
heory of COVID-19 pathogenesis Publication date: November 2020Source: Medical Hypotheses, Volume 144Author(s): Yuichiro J. Suzuki ScienceD...
-
Alimentary Pharmacology &Therapeutics, EarlyView. https://ift.tt/2qECBIJ
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου
Σημείωση: Μόνο ένα μέλος αυτού του ιστολογίου μπορεί να αναρτήσει σχόλιο.