Background. Chaperones and their co-factors are components of a cellular network; they collaborate to maintain proteostasis under normal and harmful conditions. In particular, hsp70 family members and their co-chaperones are essential to repair damaged proteins. Co-chaperones are present in different subcellular compartments, where they modulate chaperone activities.Methods and Results. Our studies assessed the relationship between hsc70 and its co-factor HspBP1 in human cancer cells. HspBP1 promotes nucleotide exchange on hsc70, but has also chaperone-independent functions. We characterized the interplay between hsc70 and HspBP1 by quantitative confocal microscopy combined with automated image analyses and statistical evaluation. Stress and the recovery from insult changed significantly the subcellular distribution of hsc70, but had little effect on HspBP1. Single-cell measurements and regression analysis revealed that the links between the chaperone and its co-factor relied on (i) the physiological state of the cell and (ii) the subcellular compartment. As such, we identified a linear relationship and strong correlation between hsc70 and HspBP1 distribution in control and heat-shocked cells; this correlation changed in a compartment-specific fashion during the recovery from stress. Furthermore, we uncovered significant stress-induced changes in the colocalization between hsc70 and HspBP1 in the nucleus and cytoplasm.Discussion. Our quantitative approach defined novel properties of the co-chaperone HspBP1 as they relate to its interplay with hsc70. We propose that changes in cell physiology promote chaperone redistribution and thereby stimulate chaperone-independent functions of HspBP1.
from #Medicine via ola Kala on Inoreader http://ift.tt/1MqK12g
via IFTTT
Αρχειοθήκη ιστολογίου
-
►
2023
(138)
- ► Φεβρουαρίου (74)
- ► Ιανουαρίου (64)
-
►
2022
(849)
- ► Δεκεμβρίου (61)
- ► Σεπτεμβρίου (74)
- ► Φεβρουαρίου (65)
-
►
2021
(2936)
- ► Δεκεμβρίου (59)
- ► Σεπτεμβρίου (180)
- ► Φεβρουαρίου (325)
-
►
2020
(1624)
- ► Δεκεμβρίου (293)
- ► Σεπτεμβρίου (234)
- ► Φεβρουαρίου (28)
-
►
2019
(13362)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (5586)
- ► Ιανουαρίου (5696)
-
►
2018
(66471)
- ► Δεκεμβρίου (5242)
- ► Σεπτεμβρίου (5478)
- ► Φεβρουαρίου (4835)
- ► Ιανουαρίου (5592)
-
►
2017
(44259)
- ► Δεκεμβρίου (5110)
- ► Σεπτεμβρίου (5105)
-
►
2016
(7467)
- ► Δεκεμβρίου (514)
- ► Σεπτεμβρίου (1038)
- ► Φεβρουαρίου (793)
-
▼
2015
(2119)
-
▼
Δεκεμβρίου
(940)
-
▼
Δεκ 21
(50)
- Molecules, Vol. 20, Pages 22900-22907: Guignardone...
- Highly-sensitive organophosphorus pesticide biosen...
- Differential cellular localization of Epstein–Barr...
- Effect of sanitation and water treatment on intest...
- C-reactive protein level is an indicator of the ag...
- Methotrexate for the management of Crohn's disease...
- Diaphragmatic breathing for rumination syndrome: e...
- Efficacy and safety of alternating norfloxacin and...
- Hepatitis C virus NS4B can suppress sting accumula...
- Nonalcoholic fatty liver disease is associated wit...
- Virtual chromoendoscopy improves the diagnostic yi...
- Assessment of energy requirements in patients with...
- HBV-specific and global T-cell dysfunction in chro...
- Sorafenib suppresses the epithelial-mesenchymal tr...
- Systematic review with meta-analysis: the diagnost...
- Potential coeliac disease markers and autoimmunity...
- A 12-week treatment with boceprevir, peginterferon...
- The Comparison of WHOQOL-BREF with Disease Specifi...
- Systemic and intestinal levels of factor XIII-A: t...
- Facial flushing response to alcohol and the risk o...
- Quantitative detection and genotyping of Helicobac...
- Percutaneous real-time sonoelastography as a non-i...
- The Monte Carlo validation framework for the discr...
- Use of Raman optical tweezers for cell cycle analysis
- Protective Effects of Streblus asper Leaf Extract ...
- Interferon gamma effect on immune mediator product...
- Larval trematode infections in Lymnaea glabra popu...
- A Novel Statistical Approach for Brain MR Images S...
- The Effect of Perioperative Ischemia and Reperfusi...
- A fluorescent and colorimetric probe containing ox...
- Sensors, Vol. 15, Pages 32138-32151: Fatigue Crack...
- Sensors, Vol. 15, Pages 32123-32137: Focusing Bist...
- Sensors, Vol. 15, Pages 32152-32167: A TSR Visual ...
- The Consumption of Bicarbonate-Rich Mineral Water ...
- Cancer: a global concern that demands new detectio...
- Factors influencing polyelectrolyte-aptamer multil...
- Orphan nuclear receptor NR4A2 inhibits hepatic ste...
- Genome-wide identification of hypoxia-induced enha...
- A novel method to determine perineal artery occlus...
- Artificial neural network cascade identifies multi...
- Quantitative analysis of the interplay between hsc...
- Digital preparation and osteology of the skull of ...
- Supplemental description and molecular characteriz...
- Molecular identification of the Cryptosporidium de...
- Ivermectin treatment of bovine psoroptic mange: ef...
- Corrigendum to “Low-Level Laser Stimulation on Adi...
- Zearalenone Inhibits Rat and Human 11β-Hydroxyster...
- The Skin as an Early Expression of Malignancies in...
- Somatosensory and Brainstem Auditory Evoked Potent...
- Effects of early adolescent environmental enrichme...
-
▼
Δεκ 21
(50)
-
▼
Δεκεμβρίου
(940)
Αναζήτηση αυτού του ιστολογίου
Δευτέρα 21 Δεκεμβρίου 2015
Quantitative analysis of the interplay between hsc70 and its co-chaperone HspBP1
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
-
Αλέξανδρος Γ. Σφακιανάκης Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,0030693260717...
-
heory of COVID-19 pathogenesis Publication date: November 2020Source: Medical Hypotheses, Volume 144Author(s): Yuichiro J. Suzuki ScienceD...
-
Alimentary Pharmacology &Therapeutics, EarlyView. https://ift.tt/2qECBIJ
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου
Σημείωση: Μόνο ένα μέλος αυτού του ιστολογίου μπορεί να αναρτήσει σχόλιο.