LLG-3 is a ganglioside isolated from the starfish Linchia laevigata. To clarify the structure-activity relationship of the glycan of LLG-3 toward rat pheochromocytoma PC12 cells in the presence of nerve growth factor, a series of mono- to tetrasaccharide glycan derivatives were chemically synthesized and evaluated in vitro. The methyl group at C8 of the terminal sialic acid residue was crucial for neuritogenic activity, and the terminal trisaccharide moiety was the minimum active motif. Furthermore, the trisaccharide also stimulated neuritogenesis in human neuroblastoma SH-SY5Y cells via mitogen-activated protein kinase (MAPK) signaling. Phosphorylation of extracellular signal-regulated kinase (ERK) 1/2 was rapidly induced by adding 1 or 10 nM of the trisaccharide. The ratio of phosphorylated ERK to ERK reached a maximum 5 min after stimulation, and then decreased gradually. However, the trisaccharide did not induce significant Akt phosphorylation. These effects were abolished by pretreatment with the MAPK inhibitor U0126, which inhibits enzymes MEK1 and MEK2. In addition, U0126 inhibited the phosphorylation of ERK 1/2 in response to the trisaccharide dose-dependently. Therefore, we concluded that the trisaccharide promotes neurite extension in SH-SY5Y cells via MAPK/ERK signaling, not Akt signaling.
from #Medicine via ola Kala on Inoreader http://ift.tt/1XHKhWI
via IFTTT
Αρχειοθήκη ιστολογίου
-
►
2023
(138)
- ► Φεβρουαρίου (74)
- ► Ιανουαρίου (64)
-
►
2022
(849)
- ► Δεκεμβρίου (61)
- ► Σεπτεμβρίου (74)
- ► Φεβρουαρίου (65)
-
►
2021
(2936)
- ► Δεκεμβρίου (59)
- ► Σεπτεμβρίου (180)
- ► Φεβρουαρίου (325)
-
►
2020
(1624)
- ► Δεκεμβρίου (293)
- ► Σεπτεμβρίου (234)
- ► Φεβρουαρίου (28)
-
►
2019
(13362)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (5586)
- ► Ιανουαρίου (5696)
-
►
2018
(66471)
- ► Δεκεμβρίου (5242)
- ► Σεπτεμβρίου (5478)
- ► Φεβρουαρίου (4835)
- ► Ιανουαρίου (5592)
-
►
2017
(44259)
- ► Δεκεμβρίου (5110)
- ► Σεπτεμβρίου (5105)
-
►
2016
(7467)
- ► Δεκεμβρίου (514)
- ► Σεπτεμβρίου (1038)
- ► Φεβρουαρίου (793)
-
▼
2015
(2119)
-
▼
Δεκεμβρίου
(940)
-
▼
Δεκ 04
(44)
- Morphology, chemical composition and nanostructure...
- Evaluation of performance reference compounds (PRC...
- Novel behavior of the chromatographic separation o...
- Surface-imprinted magnetic particles for highly se...
- Optimized efficiency of mapping a site contaminate...
- Ultrasensitive electrospun fluorescent nanofibrous...
- Abdel El Manira elected in the Royal Swedish Acade...
- Sensors, Vol. 15, Pages 30736-30758: Portable Nano...
- Sensors, Vol. 15, Pages 30716-30735: The Bubble Bo...
- Water, Vol. 7, Pages 6931-6945: Uncertainty Analys...
- Water, Vol. 7, Pages 6910-6930: Modeling Typhoon‐I...
- Sensors, Vol. 15, Pages 30759-30783: A Hybrid Indo...
- Pathogens, Vol. 4, Pages 869-882: Coordinated Mole...
- Sustainability, Vol. 7, Pages 16260-16272: Hydroge...
- IJERPH, Vol. 12, Pages 15486-15497: Influenza Vacc...
- Beverages, Vol. 1, Pages 341-353: Performance of H...
- Mouse Models of DNA Mismatch Repair in Cancer Rese...
- Characterization of γδ T-cells via flow cytometry
- Remote Sensing, Vol. 7, Pages 16519-16536: The Spa...
- IJERPH, Vol. 12, Pages 15470-15485: “Grandma, You ...
- Remote Sensing, Vol. 7, Pages 16504-16518: The Man...
- Sensors, Vol. 15, Pages 30704-30715: Preconcentrat...
- Marine Drugs, Vol. 13, Pages 7285-7300: Isolation ...
- Marine Drugs, Vol. 13, Pages 7275-7284: Biotechnol...
- Sensors, Vol. 15, Pages 30693-30703: Corrosion Res...
- Remote Sensing, Vol. 7, Pages 16480-16503: A Hybri...
- Remote Sensing, Vol. 7, Pages 16460-16479: Predict...
- Remote Sensing, Vol. 7, Pages 16441-16459: Tempora...
- Energies, Vol. 8, Pages 13879-13893: Power Transmi...
- Marine Drugs, Vol. 13, Pages 7250-7274: Structure-...
- Energies, Vol. 8, Pages 13864-13878: Combustion an...
- Anatomy Of The Supraspinatus Muscle - Everything Y...
- Screening of HER2 Overexpressed Breast Cancer Subt...
- Solvatochromic Dyes as pH-Independent Indicators f...
- Real Time Online Correction of Mass Shifts and Int...
- THE TEAM From GOOD To GREAT! - Dr. Nabil Ebraheim
- Acute restraint stress induces specific changes in...
- Momordin Ic couples apoptosis with autophagy in hu...
- Nasal Wipes for Influenza A Virus Detection and Is...
- Exercise Protocol of Multisensory Rehabilitation i...
- Effect of Triple Combination of Induction, Concurr...
- Histology-Guided High-Resolution Matrix-Assisted L...
- Methods to Investigate the Regulatory Role of Smal...
- In Situ Mapping of the Mechanical Properties of Bi...
-
▼
Δεκ 04
(44)
-
▼
Δεκεμβρίου
(940)
Αναζήτηση αυτού του ιστολογίου
Παρασκευή 4 Δεκεμβρίου 2015
Marine Drugs, Vol. 13, Pages 7250-7274: Structure-Activity Relationship Study of the Neuritogenic Potential of the Glycan of Starfish Ganglioside LLG-3 ‡
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
-
Αλέξανδρος Γ. Σφακιανάκης Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,0030693260717...
-
heory of COVID-19 pathogenesis Publication date: November 2020Source: Medical Hypotheses, Volume 144Author(s): Yuichiro J. Suzuki ScienceD...
-
https://ift.tt/2MQ8Ai8
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου
Σημείωση: Μόνο ένα μέλος αυτού του ιστολογίου μπορεί να αναρτήσει σχόλιο.